Application of inteins in building protein affinity purification system / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1175-1184, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-310550
ABSTRACT
Intein is a part of polypeptide in the premature protein with the capability of self-splicing, which is widely applied in protein purification, protein conjuction, cyclopeptide preparation, protein labeling and biosensor. In this review, we summarized the development of intein used in protein purification, discussed intein-mediated chromatographic and non-chromatographic purification systems, and summarized the researches in manipulating intein cleavage reaction. This work is to provide clues for improvement of intein-mediated protein purification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proteins
/
Chromatography, Affinity
/
Protein Splicing
/
Inteins
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article
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