Interleukin-8 inhibits clonal expansion of 3T3-L1 preadipocyte during differentiation / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 243-247, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-310755
ABSTRACT
<p><b>AIM</b>To investigate the effect of interleukin-8 (IL-8) on the differentiation and clonal expansion of 3T3-L1 preadipocyte during the differentiation period.</p><p><b>METHODS</b>The morphological changes of 3T3-L1 cells during differentiation after the treatment of IL-8 was observed by Oil-Red O staining. Glycerol-3-phosphate dehydrogenase (GPDH) activity was measured by a spectrophotometric method. MTT method and 3H-TdR incorporation were applied to examine the changes of cell proliferation and DNA synthesis in clonal expansion of 3T3-L1 cells. Cell cycle analysis was taken by flow cytometry.</p><p><b>RESULTS</b>IL-8 could inhibit the differentiation and GDPH activity in a dose dependent manner. IL-8 decreased the cell proliferation and DNA synthesis in clonal expansion after induction. Also, the proportion of cells in G1 phase was increased and that of cells in S and G2 phase was declined after the treatment of IL-8.</p><p><b>CONCLUSION</b>IL-8 inhibits the differentiation of 3T3-L1 preadipocytes by decreasing the clonal expansion of the cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Cycle
/
Cell Differentiation
/
Interleukin-8
/
Adipocytes
/
Cell Biology
/
3T3-L1 Cells
/
Cell Proliferation
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2008
Type:
Article
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