Mechanism of linoleic acid on the expression of plasminogen activator inhibitor type-1 in HepG2 cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 133-138, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-310784
ABSTRACT
<p><b>AIM</b>To investigate the molecular mechanism underlying the effect of linoleic acid on plasminogen activator inhibitor type-1 (PAI-1) expression in HepG2 cells.</p><p><b>METHODS</b>HepG2 cells were exposed to different concentrations of linoleic acid and PAI-1 expression was determined by RT-PCR and colorimetric assay. Luciferase reporter gene plasmids containing four sequentially truncated fragments of the PAI-1 promoter region (-804 to +17) were constructed, and plasmids carrying constructs of Smad binding element (SBE)-site directed deletions in PAI-1 promoter were also generated using overlap extention PCR and transiently transfected into HepG2 cells, the transcriptional activity of PAI-1 was demonstrated by the luciferase activity.The effect of linoleic acid on Smad3 and Smad4 protein levels in cultured HepG2 cells was measured by Western blot analysis.</p><p><b>RESULTS</b>(1) Linoleic acid remarkably increased PAI-1 mRNA expression and transcription in varying concentrations. (2) The level of PAI-1 transcription was gradually decreased induced by linoleic acid when transfected the SBE- site directed-deletions plasmids in PAI-1 promoter at -734/-731. (3) Protein levels of both Smad3 and 4 in HepG2 cells were increased by linoleic acid.</p><p><b>CONCLUSION</b>Linoleic acid regulated the expression of PAI-1 from transcriptional level in HepG2 cells and SBE involved in the regulation, and both Smads protein and Smad signaling pathway acted main role in this procession.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Signal Transduction
/
Gene Expression Regulation
/
Promoter Regions, Genetic
/
Plasminogen Activator Inhibitor 1
/
Linoleic Acid
/
Smad Proteins
/
Hep G2 Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2008
Type:
Article
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