Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 505-508, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-310823
ABSTRACT
<p><b>AIM</b>To improve specificity and accuracy of endogenous ouabain measurement assay.</p><p><b>METHODS</b>Anti-ouabain polyclonal antibody egg yolk (IgY) and anti-ouabain rabbit antibody (IgG) were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay (ELISA) were compared.</p><p><b>RESULTS</b>The ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%, and the inter-assay CV was 2.34% respectively. In contrast, IgG were 2.83% and 3.29%. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs. 5.95%, 3.20% vs. 5.20% of crossreaction with cedilanid and digoxin.</p><p><b>CONCLUSION</b>The specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ouabain
/
Immunoglobulin G
/
Immunoglobulins
/
Enzyme-Linked Immunosorbent Assay
/
Chickens
/
Cross Reactions
/
Egg Yolk
/
Allergy and Immunology
/
Methods
/
Antibody Specificity
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2007
Type:
Article
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