Application of rapid PCR to authenticate medicinal snakes / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3673-3677, 2014.
Article
in Zh
| WPRIM
| ID: wpr-311010
Responsible library:
WPRO
ABSTRACT
To obtained an accurate, rapid and efficient method for authenticate medicinal snakes listed in Chinese Pharmacopoeia (Zaocysd humnades, Bungarus multicinctus, Agkistrodon acutus), a rapid PCR method for authenticate snakes and its adulterants was established based on the classic molecular authentication methods. DNA was extracted by alkaline lysis and the specific primers were amplified by two-steps PCR amplification method. The denatured and annealing temperature and cycle numbers were optimized. When 100 x SYBR Green I was added in the PCR product, strong green fluorescence was visualized under 365 nm UV whereas adulterants without. The whole process can complete in 30-45 minutes. The established method provides the technical support for authentication of the snakes on field.
Full text:
1
Index:
WPRIM
Main subject:
Snakes
/
China
/
Polymerase Chain Reaction
/
Classification
/
DNA Primers
/
Reptilian Proteins
/
Genetics
/
Methods
Limits:
Animals
Country/Region as subject:
Asia
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article