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Generation of recombinant human tissue factor pathway inhibitor in E. coli / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 314-317, 2003.
Article in Chinese | WPRIM | ID: wpr-311045
ABSTRACT
Tissue factor pathway inhibitor (TFPI) is one of the major physiological inhibitors of the human blood coagulation cascade and may have great potential in the prevention and therapy of diseases caused by thrombus formation. In this study, recombinant human tissue factor was generated in E. coli containing a recombinant vector constructed by inserting TFPI cDNA into pGEX-2T vector. The generated recombinant TFPI (rTFPI) could be simply purified with glutathione-agarose affinity method and maintained its biological function in terms of inhibition of tissue factor and factor Xa.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptide Fragments / Pharmacology / Plasmids / Recombinant Proteins / Transfection / Escherichia coli / Genetics / Lipoproteins / Metabolism Limits: Humans Language: Chinese Journal: Journal of Biomedical Engineering Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptide Fragments / Pharmacology / Plasmids / Recombinant Proteins / Transfection / Escherichia coli / Genetics / Lipoproteins / Metabolism Limits: Humans Language: Chinese Journal: Journal of Biomedical Engineering Year: 2003 Type: Article