Somatic mutations of VHL gene and HIF-1alpha expression in primary renal clear cell carcinomas / 中华外科杂志
Chinese Journal of Surgery
;
(12): 196-200, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-311124
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the significance of somatic mutations of VHL gene and hypoxia-inducible factor-1alpha (HIF-1alpha) expression in primary renal clear cell carcinoma (RCC).</p><p><b>METHODS</b>Mutation of VHL gene and HIF-1alpha expression were detected by means of PCR, denaturing high-performance liquid chromatography (DHPLC), direct sequencing and immunohistochemistry in 32 samples from primary renal clear cell carcinoma patients.</p><p><b>RESULTS</b>In 32 RCC samples, 17 samples (53.1%) had and 32 samples of adjacent nonmalignant renal tissue had not mutations of VHL gene expression. Twelve RCC samples (70.6%) which had mutations of VHL gene expressed HIF-1alpha, and it had significant difference to 4 RCC (26.7%) samples which didn't have mutations of VHL gene (P < 0.05).</p><p><b>CONCLUSION</b>Mutations of VHL gene may play a significant role in the tumorigenesis of RCC, and HIF-1alpha expression correlates with it.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Transcription Factors
/
Immunohistochemistry
/
Carcinoma, Renal Cell
/
Gene Expression Regulation, Neoplastic
/
Chemistry
/
Polymerase Chain Reaction
/
Chromatography, Liquid
/
Adenocarcinoma, Clear Cell
/
Tumor Suppressor Proteins
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2004
Type:
Article
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