Gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells / 中华医学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To study the gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells.</p><p><b>METHODS</b>Total RNA extracted from human bone marrow derived mesenchymal stem cells and tendon cells underwent reverse transcription, and the products were labeled with alpha-(32)P dCTP. The cDNA probes of total RNA were hybridized to cDNA microarray with 1176 genes, and then the signals were analyzed by Atlas Image analysis software Version 1.01a.</p><p><b>RESULTS</b>Fifteen genes associated with cell proliferation and signal transduction were up-regulated, and one gene that takes part in cell-to-cell adhesion was down-regulated in tendon cells.</p><p><b>CONCLUSION</b>The 15 up-regulated and one down-regulated genes may be beneficial to the orientational differentiation of mesenchymal stem cells into tendon cells.</p>