Analysis of the response factors of different aminoglycoside antibiotics detected by evaporative light-scattering detector / 药学学报

ABSTRACT

<p><b>AIM</b>To analyze if the response factors of different aminoglycoside antibiotics detected by evaporative light-scattering detector (ELSD) are the same. If they are, then ELSD can be applied to the quality analysis of this class of antibiotics.</p><p><b>METHODS</b>The response factors of five different aminoglycosides (amikacin, sisomicin, netilmicin, etimicin and vertilmicin) detected by ELSD were determined by using a Diamonsil C18 column (150 mm x 4.6 mm, 5 microns) as analytical column and 0.2 mol.L-1 trifluoroacetic acid-methanol (946) as mobile phase at a flow rate of 0.6 mL.min-1, the temperature of the drift tube was set at 110 degrees C, and the flow of carrier gas at 2.80 L.min-1. Detector responses (A) and the amount of injection of each substance (m) were fitted to the logarithmic regression logA = blogm + loga.</p><p><b>RESULTS</b>The linear regression equation obtained were amikacin Y = 1.46X + 5.07, gamma = 0.9997; sisomicin Y = 1.51X + 5.03, gamma = 0.9997; netilmicin Y = 1.52X + 4.88, gamma = 1.000; etimicin Y = 1.46X + 4.85, gamma = 0.9999; vertilmicin Y = 1.41X + 4.90, gamma = 0.9998. The differences between them were negligible.</p><p><b>CONCLUSION</b>Different aminoglycosides can give the same responses with ELSD detection. So, the HPLC-ELSD methods can be applied to the analysis of impurities, the control of the ratio of multi-components drug and the determination of new substances by using another substance as reference, etc.</p>