Effects of chronic exposure to beta-amyloid-peptide25-35 on the mRNA expressions of voltage-gated outward potassium channel subunits in cultured rat hippocampal neurons / 药学学报
Acta Pharmaceutica Sinica
;
(12): 598-602, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-312073
ABSTRACT
<p><b>AIM</b>To investigate mRNA expression changes of voltage-gated outward potassium channel subtypes in cultured rat hippocampal neurons after chronic exposure to beta-amyloid-petitde25-35 (beta-AP25-35).</p><p><b>METHODS</b>mRNA expression was detected by RT-PCR, comparative expression levels were determined by imaging densitometer.</p><p><b>RESULTS</b>Delayed rectifying (Kv2.1, Kv1.5), transient outward (Kv1.4, Kv4.2) and large conductance calcium-activated (rSlo) potassium channel mRNA were expressed in cultured rat hippocampal. In the presence of beta-AP25-35 3 mumol.L-1 for 24 h, the relative expression level of Kv2.1 was significantly increased (n = 3, P < 0.05); the other subtypes were not changed obviously (n = 3, P > 0.05). The increase of Kv2.1 mRNA mainly happened between 24 and 36 h after exposure to beta-AP25-35. After exposure to beta-AP25-35 for 60 h, Kv2.1 mRNA decreased significantly (n = 3, P < 0.01).</p><p><b>CONCLUSION</b>The upregulation of Kv2.1 on transcription levels may be involved in the enhancement of delayed rectifying outward potassium (Ik) current induced by beta-AP25-35.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
RNA, Messenger
/
Potassium Channels
/
Gene Expression
/
Cell Division
/
Cells, Cultured
/
Amyloid beta-Peptides
/
Rats, Wistar
/
Cell Biology
/
Potassium Channels, Voltage-Gated
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2002
Type:
Article
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