Proteolysis induce factor in the digestive systematic cancer cachexia patients: its expression and role in cancer cachexia / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 1287-1290, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-312305
ABSTRACT
<p><b>OBJECTIVE</b>To demonstrate the expression of proteolysis induce factor(PIF) in the gastrointestinal(GI) cancer cachexia patients and evaluate its role in cancer cachexia.</p><p><b>METHODS</b>Examination of PIF was performed in urine samples from 28 GI cancer cachexia patients, 13 GI cancer patients without cachexia, and 12 weight loss patients with benign disease. PIF was added to the mice cultured C2C12 muscle cells, then the protein kinase B(Akt) phosphorylation and morphological change were measured.</p><p><b>RESULTS</b>The positive rate of PIF in urine of 28 cancer cachexia patients was 53.6%(15/28). In the other two groups, no positive result was detected. PIF could successfully induce Akt phosphorylation, cell atrophy, metamorphosis, and death. The peak of this phosphorylation could be detected after half an hour of the initiation of PIF at a concentration of 4 nmol/L.</p><p><b>CONCLUSIONS</b>PIF is specifically and highly expressed in GI cancer cachexia patients' urine. PIF can induce cancer cachexia possibly by activating Akt phosphorylation and inducing downstream proteolysis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proteoglycans
/
Cachexia
/
Cell Line
/
Proteolysis
/
Gastrointestinal Neoplasms
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2012
Type:
Article
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