Influence of liposome induced gene transfection on the characteristics of human immature dendritic cells / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of liposome induced gene transfection on the phenotypic characteristics and immune function of human immature dedritic cells (imDC).</p><p><b>METHODS</b>Monocytes were isolated from human cord blood, and they were differentiated into imDC by rhGM-CSF and rhIL-4 induction. Then the morphologic observation and immune phenotypic identification were performed in im-DCs. imDCs were divided into transfection (T, with liposome transfection of pEGFP vector) and control (C, without transfection) groups. The transfection rate and expression of cell maturation marker (CD83, CD86 and HLA-DR) were determined with flow cytometry, and the proliferation of non-sensitized T lymphocyte before and after transfection was determined with allogeneic mixed leukocyte reaction (MLR).</p><p><b>RESULTS</b>im-DC derived from human cord blood cells had typical appearance and surface markers consistent to what reported in the literature. The expression rates of CD86, CD83 and LA-DR in T group were (12 +/- 6) %, (8.6 +/- 2.3) % and (71 +/- 7) %, respectively, which exhibited no difference compared with those in C group (13 +/- 6) %, (9.1 +/- 3.8) % and (72 +/- 8) %, (P > 0.05). MLR results indicated that there was no obvious change in the immune stimulation function of imDC after transfection (P > 0.05) , with stimulation index lower than 2.</p><p><b>CONCLUSION</b>There is no change in maturation of imDC after liposome transfection, but the transfection efficiency needs to be elevated.</p>