miR-128a is up-regulated in hepatocellular carcinoma and promotes tumor cell proliferation by targeting RND3 / 南方医科大学学报
Journal of Southern Medical University
; (12): 1408-1413, 2014.
Article
in Zh
| WPRIM
| ID: wpr-312561
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of microRNA-128a (miR-128a) and its role in hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Nineteen pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-128a expression using qRT-PCR. A miR-128a mimics or inhibitor was transfected into HCC cells, and the cell viability was analyzed by MTT assay. RND3, one of the potential targets of miR-128a, was predicted by bioinformatics software and demonstrated by dual luciferase reporter assay. The expression of RND3 after transfection was detected using qRT-PCR and Western blotting, and the cell cycle-related proteins were determined with Western blotting.</p><p><b>RESULTS</b>The expression of miR-128a were significantly up-regulated in HCC tissues as compared to the adjacent tissues (P<0.05). In cultured HCC cells, miR-128a promoted the cell proliferation and resulted in down-regulated RND3 mRNA and protein expressions by targeting RND3' 3'UTR (P<0.05) and also in the down-regulation of cyclin B1, cyclin D1 and CDK4 protein expressions.</p><p><b>CONCLUSION</b>miR-128a is up-regulated in HCC and promotes HCC cell proliferation by targeting RND3.</p>
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
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Tumor Cells, Cultured
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Down-Regulation
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Transcriptional Activation
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Up-Regulation
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Cell Survival
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Carcinoma, Hepatocellular
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Rho GTP-Binding Proteins
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MicroRNAs
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Cell Proliferation
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article