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Effect of Yishen Daluo Decoction on the expression of PLP, Olig1, and Olig2 in mice with experimental autoimmune encephalomyelitis / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 562-565, 2014.
Article in Chinese | WPRIM | ID: wpr-312776
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Yishen Daluo Decoction (YDD) on the expression of protein lipoprotein (PLP), oligodendrocyte transcription factor 1 (Olig 1), and oligodendrocyte transcription factor 2 (Olig2) in mice with experimental autoimmune encephalomyelitis (EAE).</p><p><b>METHODS</b>Totally 40 mice were randomly divided into 4 groups, i.e., the normal group, the model group, the Chinese medicine (CM) group, and the Western medicine (WM) group, 10 mice in each group. Each mouse in the model, CM, and WM groups was subcutaneously injected with 200 microL antigen emulsion (containing 150 micro g PLP139 -151 and 400 micro g H37RA) in two parts at the upper abdomen on the first day. 100 microLBordetella pertussis juice (containing 0. 6 x 10(6) Bordetella pertussis) was injected by caudal vein on the first and the third day. On the 7th day after modeling, each mouse in the normal group and the model group was intragastrically given normal saline (0. 1 mL/10 g). YDD (0. 2 g crude drug/10 g) was intragastrically given to mice in the CM group, and prednisone (0. 039 mg/10 g) was intragastrically given to mice in the WM group. All mice were intervened for 54 days. Changes of PLP, Olig1, and Olig2 in the brain tissue of EAE mice were detected by Western blot. Results The levels of PLP and Olig2 in the brain tissue of the model group were less than those of the normal group (P <0.05). Compared with the model group, the levels of PLP, Olig1, and Olig2 in the brain tissue increased in the CM group (P <0.05); the levels of PLP and Olig2 in the brain tissue increased in the WM group (P <0.05). Compared with the WM group, the level of Olig1 in the brain tissue increased in the CM group (P <0.05).</p><p><b>CONCLUSION</b>YDD could enhance remyelination by elevating the levels of Olig1 and Olig2 in the brain tissue of EAE mice.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Transcription Factors / Brain / Drugs, Chinese Herbal / Gene Expression / Encephalomyelitis, Autoimmune, Experimental / Basic Helix-Loop-Helix Transcription Factors / Oligodendrocyte Transcription Factor 2 / Metabolism / Nerve Tissue Proteins Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Transcription Factors / Brain / Drugs, Chinese Herbal / Gene Expression / Encephalomyelitis, Autoimmune, Experimental / Basic Helix-Loop-Helix Transcription Factors / Oligodendrocyte Transcription Factor 2 / Metabolism / Nerve Tissue Proteins Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2014 Type: Article