E. coli-based production of recombinant FALL-39 / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 634-637, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-312909
ABSTRACT
This study was aimed at constructing a prokaryotic expression system to resolve the difficulties in acquiring antibacterial peptide and to meet the needs of research and drug development. Total RNA was extracted from human pulmonary gland epithelial cell line SPC-A-1, and a cDNA encoding mature FALL-39 peptide was amplified by RT-PCR. The recombinant prokaryotic expression vector pGEX-1 lambda T-FALL-39 was constructed. Using affinity chromatography, thrombin cleaving and AU-PAGE elution, we obtained the purified FALL-39. MIC, MEC, MBC analyses demonstrated that the FALL-39 had strong antibacterial activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Biosynthesis
/
Peptides
/
Pharmacology
/
Recombinant Proteins
/
Escherichia coli
/
Genetic Vectors
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2003
Type:
Article
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