Effect of precondition with GBE50 and Salviae miltionrrhizae on cycloxygenase-2 and its downstream effectors contents in ischemia/reperfusion myocardium / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 1056-1060, 2010.
Article
in Zh
| WPRIM
| ID: wpr-313163
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in contents of cycloxygenase-2 (COX-2) and its downstream effectors in rat's myocardial ischemia/reperfusion (I/R) model and observe the effects of precondition with GBE50 (Ginkgo biloba extract 50) and Salviae miltiorrhizae (SM) on them.</p><p><b>METHODS</b>Rat's I/R model was established by 30-min left anterior descending coronary artery occlusion followed with 60-min reperfusion. Animals were divided into the model control group, the sham-operated group and the tested groups (received 1-week precondition with GBE50 and SM respectively via intragastric infusion before modeling). COX-2 mRNA expression in myocardium was detected by real-time PCR; contents of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) were measured by radioimmunoassay.</p><p><b>RESULTS</b>The mRNA expression of COX-2 in the model group was obviously higher than that in the sham-operated group (P < 0.001), while that in the tested groups was down-regulated significantly (P < 0.01), and the content of TXB2 as well as the ratio of TXB2/PGF1alpha was reduced significantly (P < 0.05). Besides, SM also showed the up-regulation effect on 6-keto-PGF1alpha content in myocardium (P < 0.05).</p><p><b>CONCLUSION</b>COX-2 affects the myocardium through thromboxane A2 and prostacyclin after I/R; both GBE50 and SM can inhibit the production of COX-2, but they may act in different paths.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Thromboxane B2
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RNA, Messenger
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Drugs, Chinese Herbal
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6-Ketoprostaglandin F1 alpha
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Myocardial Reperfusion Injury
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Chemistry
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Rats, Sprague-Dawley
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Myocardial Ischemia
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Ischemic Preconditioning, Myocardial
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2010
Type:
Article