Effects of changwelqing on nuclear translocation of Y-box binding protein-1 and expression of P-glycoprotein in human colon cancer cell line with drug-resistance induced by vincristine / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 743-747, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-313207
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects and molecular mechanism of action of Changweiqing (CWQ) in reversing multidrug resistance by observing its impacts on nuclear translocation of Y-box binding protein-1 (YB-1), multi-drug resistance gene (MDR1) expression and P-glycoprotein (P-gp) expression in human colon cancer cell line HCT8/V with drug-resistance induced by vincristine.</p><p><b>METHODS</b>Cultured HCT8/V cells were exposed to the CWQ-containing rat serum prepared by drug perfusion. YB-1 expressions in cell plasma and nuclei were examined by Western blot; the binding activity of YB-1 to MDR1 gene promoter sequences was detected by electrophoretic mobility shift assay (EMSA); the mRNA transcription levels of MDR1, YB-1 and multi-resistance related protein (MRP) were examined by RT-PCR; the expression of P-gp on cell membrane was determined by flow cytometry. Results Along with the increasing drug's concentration of CWQ-containing serum from 1.25% up to 2.5% and 5%, the expressions of YB-1 decreased in HCT8/V cell nuclear and increased in cytoplasm gradually; the binding activity of YB-1 to MDR1 gene promoter weakened (P < 0.01), MDR1 mRNA expression and fluorescence intensity of P-gp on cell membrane attenuated (P < 0.05 or P < 0.01), while YB-1 and MRP mRNA unchanged (P > 0.05).</p><p><b>CONCLUSION</b>CWQ could reverse the drug-resistance of colon cancer cells by influencing nuclear translocation of YB-1 and reducing the expression of MDR1/P-gp.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Vincristine
/
Drugs, Chinese Herbal
/
Colonic Neoplasms
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Neoplasm
/
Active Transport, Cell Nucleus
/
Cell Line, Tumor
/
Y-Box-Binding Protein 1
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS