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Effect of Shenqi compound on PTEN/PI3K signal transduction in GK rats with diabetes mellitus macroangiopathy / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 640-644, 2010.
Article in Chinese | WPRIM | ID: wpr-313231
ABSTRACT
<p><b>OBJECTIVE</b>To research the effects and mechanism of Shenqi compound (SQC) on PTEN/ PI3K signal transducing path and angiogenesis in Goto-Kakizaki (GK) rats with diabetes mellitus type 2 (DM2) caused macroangiopathy.</p><p><b>METHOD</b>GK rats with blood sugar > or = 11.1 mmol/L were divided into 4 groups, the GK group, the model group, the Western medicine (WM) group treated by atorvastatin 1.5 mg/(kg x d) and the Chinese medicine (CM) group treated with SQC 1.44 g/(kg x d). All were fed 35 days with high fatty diet, but to the latter three groups, N omega-nitriyl-L-arginine methyl ester 0.1 mg/mL was added into their drinking water for macroangiopathy model establishing. Besides, a group of normal Wistar rats fed with ordinary forage was set for control. Rat's blood glucose and lipids were measured, morphology of abdominal aorta wall tissue was observed with HE staining, and mRNA expressions of PTEN and PI3Kp85 in aortic wall were detected by Real-time PCR.</p><p><b>RESULTS</b>General condition, gluco-lipid metabolism and aortic morphology in the CM group were significantly better than those in the model group. PTEN mRNA expression in the CM group (1.10 +/- 0.48) was significantly higher than that in the GK group (0.63 +/- 0.16) and the model group (0.17 +/- 0.07, both P < 0.01), but near to that in the WM group (1.11 +/- 0.46), while the PI3Kp85 mRNA expression in the TCM group (0.19 +/- 0.05) was lower than that in the GK group (1.38 +/- 0.43, P < 0.01), but near to that in the model group (0.33 +/- 0.09) and the WM group (0.11 +/- 0.06, both P > 0.05).</p><p><b>CONCLUSION</b>SQC could increase the PTEN mRNA expression and restrain the PI3Kp85 mRNA expression in aorta, which is possibly the partial mechanisms of action of the remedy in inhibiting angiogenesis and preventing diabetic macroangiopathy.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Aorta / Pharmacology / Rats, Inbred Strains / RNA, Messenger / Drugs, Chinese Herbal / Signal Transduction / Chemistry / Rats, Wistar / Angiogenesis Inhibitors / Astragalus propinquus Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Aorta / Pharmacology / Rats, Inbred Strains / RNA, Messenger / Drugs, Chinese Herbal / Signal Transduction / Chemistry / Rats, Wistar / Angiogenesis Inhibitors / Astragalus propinquus Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2010 Type: Article