The Effects of Glutathione on Cyclosporine Induced Cytotoxicity in Cultured Insulinoma Cells / 대한이식학회지
The Journal of the Korean Society for Transplantation
;
: 147-150, 2001.
Article
in Korean
| WPRIM
| ID: wpr-31329
ABSTRACT
PURPOSE:
Cycloporine A (CsA) is a immunosuppressive agent most widely used in organ transplanted patients for preventing immunorejection, but it has some side effects, including hypertension, nephrotoxicity, hepatotoxicity and diabetes. The mechanism of toxicity of CsA was not completely understood, but the reactive oxygen species has been proposed to be involved in the reaction of toxicity of CsA. The purpose of this study is to determine the effects of glutathione, as a physiological antioxidant on CsA induced cytotoxicity in rat insulinoma (RINm5F) cells.METHODS:
RINm5F cells were incubated in the presence of CsA (105~108 M) and buthionine sulfoximine (BSO), as a inhibitor of r-glutamyl cysteine synthetase, was added to cultured media (RPMI1640). Twenty four hours of incubation with CsA and BSO, viable cells were determined by MTT method.RESULTS:
CsA decreased cell viability in dose response in cultured RINm5F cells and significantly decreased according to redusing glutathione.CONCLUSION:
These results suggested that CsA may induce the diabetes and glutathione have some roles in the pathogenesis of CsA-induced diabetes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Survival
/
Cyclosporine
/
Reactive Oxygen Species
/
Buthionine Sulfoximine
/
Cysteine
/
Glutathione
/
Hypertension
/
Insulinoma
/
Ligases
Limits:
Animals
/
Humans
Language:
Korean
Journal:
The Journal of the Korean Society for Transplantation
Year:
2001
Type:
Article
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