Effect of reactive oxygen species during the leukemogenic process associated with exposure to benzene / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 738-740, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-313462
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of reactive oxygen species (ROS) during the leukemogenic process associated with exposure to benzene.</p><p><b>METHODS</b>HL-60 was treated with 3 micromol/L benzoquinone (BQ). Generation of ROS in cells was measured by DCFH-DA method. For proliferation assays,cells were stained with alamar blue dye and counted.</p><p><b>RESULTS</b>ROS production and the proliferation of cell were all increased in BQ-treated cells (13.10 +/- 0.15, 185% +/- 30.00%) as compared with control cells (11.32 +/- 0.09, 100% +/- 0.00%) (P < 0.05); The addition of catalase just before BQ addition reduced ROS generation to basal levels and decreased the growth of cell (P < 0.05).</p><p><b>CONCLUSION</b>ROS may play an important role in the process of proliferation of HL-60 cells induced by BQ.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Benzoquinones
/
Reactive Oxygen Species
/
HL-60 Cells
/
Cell Proliferation
/
Toxicity
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2009
Type:
Article
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