Renin-angiotensin system in mesenteric adipose tissues in rats with metabolic syndrome / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 770-775, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-313687
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the renin-angiotensin system (RAS) in mesenteric adipose tissues and effect of angiotensin II on adipocyte differentiation.</p><p><b>METHODS</b>Thirty normal 8-week-old male Wistar rats were divided into groups on normal diet and high-fat diet. The rats on high-fat diet for 24 weeks developed the metabolic syndrome respectively. The mRNA and protein expression of mesenteric adipose tissue were measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Lipid drop in 3T3-L1 preadipocytes and mature adipocytes were observed using oil-red O staining. The fluorescence microscope was used to detect cytosolic-free calcium in 3T3-L1 preadipocytes and mature adipocytes.</p><p><b>RESULTS</b>The expressions of angiotensinogen, angiotensin converting enzyme, angiotensin II receptor type 1 in mesenteric adipose tissue were significantly increased in rats with metabolic syndrome compared with those in rats on normal diet (P <0. 05, P <0. 01). After administration of angiotensin II , no lipid droplet in 3T3 -L1 preadipocytes and adipocytes were observed, however, intensive lipid droplet in adipocyte was found after administration of captopril and candesartan. Angiotensin II increased the intracellular-free calcium concentration in preadipocytes (P < 0. 01 ) , which was blocked by captopril and candesartan; in contrast, angiotensin II effect was blunt in mature adipocyte. Captopril and candesartan partially recovered the angiotensin II -mediated increase of cytosolic-free calcium.</p><p><b>CONCLUSION</b>RAS in the mesenteric adipose tissues is active in rats with metabolic syndrome, and antagonization of RAS can recover the lipogenesis of adipocyte.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Renin-Angiotensin System
/
Tetrazoles
/
Benzimidazoles
/
RNA, Messenger
/
Angiotensin II
/
Captopril
/
Cells, Cultured
/
Angiotensinogen
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS