Resistance to HIV-1 infection of CD4 + T lymphocytes in vitro from chinese HIV-1 exposed seronegative individuals / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 613-617, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-313722
ABSTRACT
<p><b>OBJECTIVE</b>To determine the relative resistance to HIV-1 infection of CD4 + T lymphocytes in HIV-exposed seronegative individuals (ESNs) in China.</p><p><b>METHODS</b>HIV primary isolates were obtained from peripheral whole blood of HIV-infected persons. CD4 + T lymphocytes of Chinese ESNs were separated from peripheral blood mononuclear cells with magnetic cell sorting (MACS). The purified CD4 + T lymphocytes were cocultured with HIV primary isolates. The p24 level was detected and the culture medium was refreshed every 3 days within 2 weeks.</p><p><b>RESULTS</b>For M tropic HIV strains, p24 level was significantly lower in ESN group than in control group (P < 0.05); for some M tropic HIV strains, even no p24 replicated in ESN group. However, T tropic virus strains had no significant difference between these two groups (P > 0.05).</p><p><b>CONCLUSION</b>CD4 + T lymphocytes of Chinese ESNs may possess relative resistance to M tropic HIV strains, which may be one of the main influencing factors that result in ESN.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Virulence
/
In Vitro Techniques
/
Sexual Partners
/
CD4-Positive T-Lymphocytes
/
HIV Infections
/
China
/
HIV
/
Classification
/
HIV Seronegativity
Limits:
Adult
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Type:
Article
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