Preparation and evaluation of microbubble ultrasound contrast agent with N-carboxymethyl chitosan / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 497-501, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-313745
ABSTRACT
<p><b>OBJECTIVE</b>To prepare microbubble, made of N-carboxymethyl chitosan, as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo.</p><p><b>METHODS</b>Oil-Water-Oil multiple emulsion/solvent evaporation method was used to prepare the microbubble contrast agent. Both optical micrography and scanning electron micrography were performed to determine the bubble size and morphology. The acoustic effect of the N-carboxymethyl chitosan echo contrast agent was evaluated in vivo in rabbit. Liver echo images were recorded with ultrasound machine before and after intravenous bolus injecting 0.5 ml of the agent.</p><p><b>RESULTS</b>The novel N-carboxymethyl chitosan echo contrast agent was formulated as lyophilized product, with a mean diameter of 2-3 microm and a shell thickness of 250-300 nm. Its size is relatively uniform. The imaging effect was remarkably enhanced with the ultrasonic contrast agent when applied in rabbit livers.</p><p><b>CONCLUSION</b>It is feasible to prepare excellent microbubble ultrasound contrast agent with N-carboxymethyl chitosan as membrane components.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ultrasonics
/
Diagnostic Imaging
/
Chemistry
/
Chitin
/
Ultrasonography
/
Contrast Media
/
Microbubbles
/
Liver
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Type:
Article
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