Expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 805-808, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-313890
ABSTRACT
This study was aimed to investigate the expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells (MSC). Human bone marrow MSC were isolated and enriched by Fircoll density gradient centrifugation, then identified by flow cytometry. MSC were induced with dexamethasone, insulin, 3-isobutyl-1-methyl-xanthine (IBMX) and indomethacin to differentiate into adipocytes. Then the expression of survivin and HO-1 in MSC was detected by RT-PCR. The results indicated that the expressions of surface antigen CD34 and HLA-DR in MSC in vitro were negative while the expressions of CD44 and CD71 were positive. MSC could be differentiated into adipocytes by inductor. RT-PCR showed the expression of radioresistant genes survivin and HO-1 in MSC. It is concluded that MSC have lower sensitivity to radiation, which may associate with the expression of radioresistant genes survivin and HO-1 in MSC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Bone Marrow Cells
/
Gene Expression
/
Cell Differentiation
/
Cells, Cultured
/
Heme Oxygenase-1
/
Inhibitor of Apoptosis Proteins
/
Mesenchymal Stem Cells
/
Flow Cytometry
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article
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