Apoptosis-inducing effect of clostridium difficile toxin A on K562 cells and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 638-642, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-313926
ABSTRACT
This study was purposed to investigate the growth inhibition and apoptosis-inducing effect of Clostridium difficile toxin A (TcdA) on the leukemia cell line K562. The proliferative activity of K562 cells exposed to Tcd A was tested by MTT assay, cell apoptosis was detected by flow cytometry; immunocytochemistry and colorimetric assay were employed to detect the protein expressions of BCL-2/BAX and the activity of Caspase-3, respectively. The results indicated that the proliferation of K562 cells was inhibited in a time-and dose-dependent manner after exposure to Tcd A for 24, 48 and 72 hours, the cells displayed the typical apoptotic, morphological changes, the expression of BCL-2 protein was down-regulated but the expression of BAX protein was signficantly increased, compared with control group (p < 0.05). In addition, caspase-3 was activated in a concentration-dependent manner. It is concluded that Tcd A inhibits cell growth of K562 by inducing apoptosis, and the up-regulation of BAX protein and activation of caspase-3 may play important roles in these processes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bacterial Toxins
/
Gene Expression Regulation, Leukemic
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
K562 Cells
/
Enterotoxins
/
Bcl-2-Associated X Protein
/
Caspase 3
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article
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