The role of cell pathway in apoptosis of fibroblasts from proliferative scar induced by steroid or IFN / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 46-49, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-314162
ABSTRACT
<p><b>OBJECTIVE</b>This paper is to investigate the effects of steroid or IFN alpha-2b on apoptosis and cell pathway of fibroblasts from keloids, hypertrophic scars and normal skins and different responses of different fibroblasts.</p><p><b>METHODS</b>6 samples from keloid, hypertrophic scar and normal skin were collected respectively and fibroblasts from different sources were cultured in vitro. After different fibroblasts were treated with dexamethasone (0.1 mg/ml) or IFN alpha-2b (1000 U/ml), Bax and Bcl-2 protein expressions were detected in situ by immunohistochemical staining; DNA ladders of different fibroblasts were observed by gel electrophoresis; and relative activated (phospho-) ERK1/2 and JNK pathways were detected by method of FACE ELISA.</p><p><b>RESULTS</b>Dexamethasone could induce apoptosis of fibroblasts from keloids, hypertrophic scars and normal skins through activating (phospho-) ERK1/2 and JNK pathways; IFN alpha-2b could not induce apoptosis of fibroblasts from different sources. IFN alpha-2b could inhibit (phospho-) ERK1/2 pathway and could not affect (phospho-) JNK pathways of fibroblasts from keloid and hypertrophic scar. IFN alpha-2b could affect neither (phospho-) ERK1/2 pathway nor (phospho-) JNK pathways of fibroblasts from normal skin.</p><p><b>CONCLUSIONS</b>The responses of different fibroblasts to steroid or IFN alpha-2b were different.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Recombinant Proteins
/
Signal Transduction
/
Cells, Cultured
/
Interferon-alpha
/
Cicatrix, Hypertrophic
/
Apoptosis
/
Fibroblasts
/
Hormones
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2008
Type:
Article
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