Creation of tissue engineered cartilage with internal support / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 328-331, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-314221
ABSTRACT
<p><b>OBJECTIVE</b>To test the hypothesis that tissue-engineered cartilage can be bioincorporated with a nonreactive, permanent endoskeletal scaffold.</p><p><b>METHODS</b>Chondrocytes obtained from swine articular were seeded onto polyglycolic acids(PGA) scaffold which was incorporated with high-density polyethylene (Medpor). After cultured in vitro for two weeks,the cell-scaffold construct was implanted into subcutaneous pockets on the back of nude mice. Six weeks later,the newly formed cartilage prosthesis was harvested, and a small part of sample was evaluated by gross view, histology, type II collagen immunohistochemistry and biochemistry. PGA scaffold seeded with cells as the control group.</p><p><b>RESULTS</b>The newly formed cartilage was very similar to normal cartilage in both gross view and histology, and jointed Medpor tightly. The center of control group was hollow.</p><p><b>CONCLUSION</b>This pilot technique combining tissue engineering with a permanent success in creating cartilage without "hollow" phenomenon. biocompatible endoskeleton demonstrated</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyethylenes
/
Swine
/
Transplantation
/
Biocompatible Materials
/
Materials Testing
/
Cartilage
/
Pilot Projects
/
Chondrocytes
/
Cell Biology
/
Tissue Engineering
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2007
Type:
Article
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