Experimental study of increasing effect of the fat flap tissue after delay operation on its free graft survival volume and duration / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 251-253, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-314241
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of the fat flap tissues after delay operation on free fat-graft survival rate and duration.</p><p><b>METHODS</b>The delay operation of fat flaps was performed in the inguinal region of a rabbit. Expression of VEGF was assayed using Elisa method after 12 hours of flap delay. The fat flaps were harvested and cut into pieces after 21 days. A subdermal pocket was created in each side of the dorsal midline of a rabbit, the fat pieces were grafted randomly into a pocket and the normal fat pieces into the other pocket as control. After 1, 3, 6, 9 and 12 months of implantation, the grafted fats were harvested, gross observation, weight measurement and histology were carried out. Number of the vessels stained with anti-CD34 antibody was counted out.</p><p><b>RESULTS</b>VEGF concentrations in flaps were significantly higher (P < 0.05). The density of vessels in experimental groups increased significantly compared with that in control groups at 1 and 3 months, respectively (P < 0.01), and no significant differences in the survival rate of fat tissues between experimental and control groups were observed at 1 and 3 months (P > 0.05). The fat cells from the flaps survived after 12 months of fat plantation, while those in control groups disappeared after 6 months.</p><p><b>CONCLUSIONS</b>The survival rate and duration of grafted fat could be increased implanting the fat tissues from delayed fat flap, which may provide researchers with a new method for fat graft.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Surgical Flaps
/
Transplantation
/
Adipose Tissue
/
Adipocytes
/
Graft Survival
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2007
Type:
Article
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