Study on the involvement of ZO-1 gene in leukemogenesis / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 473-476, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-314459
ABSTRACT
<p><b>OBJECTIVE</b>To explore ZO-1 gene expression and methylation in leukemia cells and the involvement of ZO-1 gene in leukemogenesis.</p><p><b>METHODS</b>Restriction landmark genomic scanning (RLGS) was used to identify new leukemia related gene, and methylation specific PCR (MSP) for ZO-1 methylation status. ZO-1 specific siRNA was designed and prepared by in vitro transcription and transfected into K562 cells, the transfected cells were cultured for 48 hours before harvesting. The effect of ZO-1 siRNA was monitored by Northern blot. Cellular proliferation capacity was assayed by CCK-8, cell apoptosis by Annexin V-fluorescence in isothiocyanate (FITC) assay, and cell cycle by phosphatidylinositol (PI).</p><p><b>RESULTS</b>The intensified spots in RLGS gel were subjected to bioinformatics analysis and one of the candidate spots was proved to be ZO-1 gene. In fresh leukemia cells, Molt4 cells and HL-60 cells, ZO-1 was hypermethylated, causing it reduced or silenced. ZO-1 gene was highly expressed with no methylation in normal peripheral blood MNC and K562 cells. There was a good correlation between promoter methylation and the gene silence. The silenced gene can be re-activated by demethylation treatment with 5-AZA-dC in most leukemia cell lines. RNA interference for ZO-1 gene in K562 cells did not interfere with cell proliferation, cell cycle and apoptosis.</p><p><b>CONCLUSION</b>ZO-1 gene methylation might be involved in the tumorigenesis of acute leukemia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Phosphoproteins
/
Leukemia
/
HL-60 Cells
/
DNA Methylation
/
K562 Cells
/
Zonula Occludens-1 Protein
/
Genetics
/
Membrane Proteins
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS