Role of WAVE1 in K562 leukemia cells invasion and its mechanism / 中华血液学杂志
Chinese Journal of Hematology
; (12): 237-241, 2009.
Article
in Zh
| WPRIM
| ID: wpr-314497
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate role of WASP family verprolin homologous protein 1 (WAVE1) in K562 leukemia cell invasion and its mechanism.</p><p><b>METHODS</b>Immunofluorescence method was used to detect the distribution of WAVE1 and MMP-2 in the cells. K562 cells were transfected with pcDNA3. 1-WAVE1 reconstructed plasmid or with specific siRNA to WAVE1 gene. The invasion ability of K562 cells was examined by Transwell assay. The expression level of WAVE1 and MMP-2 in K562 cells was assayed by real-time PCR and Western blot.</p><p><b>RESULTS</b>(1) WAVE1 and MMP-2 mainly expressed and co-localized in the cell membrane; (2) 24 h and 48 h after transfected with pcDNA3. 1-WAVE1, the MMP-2 mRNA level in K562 cells increased by 295% and 198% while its protein increased by 80% and 23% respectively as compared with control K562 cells. At the same time point after transfected with specific siRNA, the MMP-2 mRNA level decreased by 81% and 28%, and its protein decreased by 36% and 53% respectively as compared with control. (3) The invasion ability of K562 cells was enhanced after transfected with pcDNA3. 1-WAVE1 and depressed after transfected with the specific siRNA.</p><p><b>CONCLUSION</b>The co-localization of WAVE1 and MMP-2 in K562 cells suggests they coordinate in functions; WAVE1 may involve in the migration and invasion of K562 cells through regulating the expression level of MMP-2.</p>
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
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Transfection
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Leukemic Infiltration
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K562 Cells
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Matrix Metalloproteinase 2
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RNA, Small Interfering
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Wiskott-Aldrich Syndrome Protein Family
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Genetics
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2009
Type:
Article