Study of influence of umbilical cord mesenchymal stem cells on CD34+ cells in vivo homing in NOD/SCID / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 103-106, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-314527
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect and the potential mechanism of umbilical cord (UC) derived mesenchymal stem cells (MSCs) on umbilical cord blood (UCB) derived CD34+ cells in vivo homing in xenotransplanted NOD/SCID mice model.</p><p><b>METHODS</b>CD34+ cells and MSCs were derived from fresh UCB and UC, respectively. CD34+ cells (5 x 10(5) per mice) and MSC cells (5 x 10(6) per mice) were co-transplanted into irradiated NOD/SCID mice intravenously. CD34+ cells (5 x 10(5) per mice) alone were transplanted into the mice as control group. CD34+ cells home in bone marrow and spleen of recipient mice were detected 20 hours after transplant by FACS and RT-PCR, and the homing efficiencies were calculated. The effect of MSCs on CD34+ cells chemotactic function was investigated after co-cultured UCB CD34+ cells with UC MSCs in vitro. After 4 and 7 days coculture, the homing related adhesion molecules (the CD49e, CD31, CD62L, CD11a) expressed on CD34+ cells were detected by FACS.</p><p><b>RESULTS</b>1) The homing efficiencies in bone marrow in experimental and control group were (7.2 +/- 1.1)% and (5.4 +/- 0.9)%, respectively (P < 0.05). 2) Human GAPDH gene was detected in bone marrow in experimental group and in spleen in both groups. 3) The migration efficiency of CD34+ cells was significantly higher in experimental group (35.7 +/- 5.8)% than in control group (3.5 +/- 0.6)% (P < 0.05). 4) The expression of CD49e, CD31, CD62L on CD34+ cells kept higher level in MSCs cocultured group than in CD34+ cells alone group.</p><p><b>CONCLUSIONS</b>MSCs can efficiently increase homing of CD34+ cells to bone marrow and spleen in vivo by keeping a high level of homing adhesion molecules expression and improving migration efficiency of UCB CD34+ cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Hematopoietic Stem Cells
/
Cell Movement
/
Cells, Cultured
/
Mice, SCID
/
Mice, Inbred NOD
/
Hematopoietic Stem Cell Transplantation
/
Coculture Techniques
/
Antigens, CD34
/
Cell Biology
/
Mesenchymal Stem Cell Transplantation
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2009
Type:
Article
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