The role of Gli1 in the invasion and migration of pancreatic cancer cells / 中华外科杂志
Chinese Journal of Surgery
;
(12): 518-522, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-314677
ABSTRACT
<p><b>OBJECTIVE</b>To study the role and possible mechanism of glioma-associated oncogene-1 (Gli1) in regulating the cell invasion and migration of pancreatic cancer cells.</p><p><b>METHODS</b>Quantitative real-time (qRT) -PCR was used to detect the effect of siRNA interference on Gli1, murine double minute 2 (MDM2) and p53 genes. Cell invasion and migration assays were used to observe the effect of Gli1, MDM2 and p53 silence on cell invasion and migration in p53 wild-type Capan-2 pancreatic cancer cells, respectively. Meanwhile, immunoblotting (IB) was used to detect the protein level of matrix metalloproteinase (MMP) -9, phospho-excelluar signal-regulated kinase (pERK) and phosphorylation protein kinase B (pAKT) in Gli1-silencing Capan-2 cells. The data were analyzed by paired t-test.</p><p><b>RESULTS</b>qRT-PCR showed that the expression of Gli1, MDM2 and p53 is down-regulated 70.5% and 74.5%, 61.8% and 65.3%, and 73.8% and 78.2% after siRNA interference, compared with the mock and siRNA control groups, respectively. Cell invasion (94 ± 8) and migration (143 ± 8) in p53 wild-type Capan-2 cells transfected with Gli1siRNA were significantly decreased, compared with the siRNA control group (150 ± 7, 190 ± 10) (t = 6.584, P = 0.022; t = 8.266, P = 0.014) , while MDM2 silence inhibited cell invasion (experiment group85 ± 12, control group 138 ± 6) and migration (experiment group 127 ± 9, control group180 ± 10) in the same cells, respectively (t = 5.097, P = 0.036;t = 4.860, P = 0.040). However, cell invasion (experiment group 153 ± 11, control group 106 ± 7) and migration (experiment group 209 ± 13, control group 164 ± 8) in p53-silencing Capan-2 cells were significantly enhanced (t = 4.669, P = 0.043; t = 4.990, P = 0.038). IB showed that Gli1 silence down-regulated MMP-9 but not pERK and pAKT protein expression.</p><p><b>CONCLUSION</b>Gli1 might contribute to the cell invasion and migration in pancreatic cancer via the regulation of MDM2, p53 and MMP-9 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Pancreatic Neoplasms
/
Pathology
/
Transfection
/
Trans-Activators
/
Cell Movement
/
Tumor Suppressor Protein p53
/
Oncogene Proteins
/
Matrix Metalloproteinase 9
/
RNA, Small Interfering
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2014
Type:
Article
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