Change of gene expression of transforming growth factor-beta1, Smad 2 and Smad 3 in hypertrophic scars skins / 中华外科杂志
Chinese Journal of Surgery
;
(12): 17-19, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-314945
ABSTRACT
<p><b>OBJECTIVE</b>To explore the regulatory mechanisms of transforming growth factor-beta1 (TGF-beta1) and two transcriptional factors Smad 2, 3 on hypertrophic scar formation and fetal scarless healing.</p><p><b>METHODS</b>Thirty-two cases were detected to compare the gene expression of TGF-beta1, Smad 2 and Smad 3 with RT-PCR. Among those cases, there were 8 cases of hypertrophic scars, 8 cases of control skins, 8 cases of fetal skins and 8 cases of adult skins.</p><p><b>RESULTS</b>TGF-beta1, Smad 2 and Smad 3 gene expression could all be detected in hypertrophic scars, fetal and adult skins. Among 8 groups examinated in this experiment (each group comprised a hypertrophic scar and its corresponding normal skin), there were 5, 8 and 5 groups in which TGF-beta1, Smad 2 and Smad 3 gene expression were higher in hypertrophic scars than in normal skins respectively. The fetal skins showed significantly lower level of TGF-beta1 and Smad 3 gene expression compared with adult skins (t = 2.204, P < 0.05 and t = 4.269, P < 0.01 respectively), while mRNA contents of Smad 2 were obviously higher in fetal skins than in adult skins (t = 6.685, P < 0.01).</p><p><b>CONCLUSION</b>TGF-beta1 and its downstream signal molecules Smad 2, Smad 3 might be involved in hypertrophic scar formation. Higher gene expression of TGF-beta1, Smad 2 and Smad 3 in hypertrophic scars might lead to stimulating extracellular matrix deposition, inducing fibroblast proliferation and accelerating fibrogenesis. Lower mRNA contents of TGF-beta1 and Smad 3 in fetal skins compared with adult skins might be associated with fetal scarless healing.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Trans-Activators
/
Gene Expression Regulation
/
Transforming Growth Factor beta
/
Cicatrix, Hypertrophic
/
Reverse Transcriptase Polymerase Chain Reaction
/
DNA-Binding Proteins
/
Smad2 Protein
/
Smad3 Protein
/
Transforming Growth Factor beta1
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2002
Type:
Article
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