Application of SPSS orthogonal design in tissue culture of Anoectochilus roxburghii / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2581-2585, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-315402
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of the different constitutions of plant hormone on the development of Anoectochilus roxburghii.</p><p><b>METHOD</b>A. roxburghii were harvested after having been cultured for 60 days. An orthogonal design was used to study the effect of NAA and 6-BA on the leaf number, eustipe number, lateral branch number of the stem tip and stem section, and the height of the stem tips. All of the data were processed by SPSS.</p><p><b>RESULT AND CONCLUSION</b>It is reported for the first time that NAA could make different development of A. roxburghii at low concentration ( < 1 mg L(-1)) and high concentration ( > 1 mg L(-1)). The optimum constitution of MS medium was NAA 0.5 mg L(-1) + 6-BA 1 mg L(-1) for the growth of the stem tip of A. roxburghii, and NAA 1 mg L(-1) + 6-BA 2 mg L(-1) for the differentiation of bud and the formation of lateral branch of the stem section. The different concentrations of NAA and 6-BA had different effects on the growth and differentiation of the stem tip and the stem section of A. roxburghii.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Growth Regulators
/
Purines
/
Benzyl Compounds
/
Software
/
Data Interpretation, Statistical
/
Orchidaceae
/
Tissue Culture Techniques
/
Kinetin
/
Metabolism
/
Naphthaleneacetic Acids
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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