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Application of SPSS orthogonal design in tissue culture of Anoectochilus roxburghii / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-315402
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of the different constitutions of plant hormone on the development of Anoectochilus roxburghii.</p><p><b>METHOD</b>A. roxburghii were harvested after having been cultured for 60 days. An orthogonal design was used to study the effect of NAA and 6-BA on the leaf number, eustipe number, lateral branch number of the stem tip and stem section, and the height of the stem tips. All of the data were processed by SPSS.</p><p><b>RESULT AND CONCLUSION</b>It is reported for the first time that NAA could make different development of A. roxburghii at low concentration ( < 1 mg L(-1)) and high concentration ( > 1 mg L(-1)). The optimum constitution of MS medium was NAA 0.5 mg L(-1) + 6-BA 1 mg L(-1) for the growth of the stem tip of A. roxburghii, and NAA 1 mg L(-1) + 6-BA 2 mg L(-1) for the differentiation of bud and the formation of lateral branch of the stem section. The different concentrations of NAA and 6-BA had different effects on the growth and differentiation of the stem tip and the stem section of A. roxburghii.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plant Growth Regulators / Purines / Benzyl Compounds / Software / Data Interpretation, Statistical / Orchidaceae / Tissue Culture Techniques / Kinetin / Metabolism / Naphthaleneacetic Acids Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2009 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plant Growth Regulators / Purines / Benzyl Compounds / Software / Data Interpretation, Statistical / Orchidaceae / Tissue Culture Techniques / Kinetin / Metabolism / Naphthaleneacetic Acids Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2009 Type: Article