Cloning and characterization of open reading frame encoding beta-amyrin synthase in Glycyrrhiza uralensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2438-2440, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-315428
ABSTRACT
<p><b>OBJECTIVE</b>To clone and sequence the open reading frame of beta-amyrin synthase (bAS) from Glycyrrhiza uralensis.</p><p><b>METHOD</b>The primers were designed according to the cDNA sequence of beta-amyrin synthase from G. glabra reported by Hiroaki HAYASHI, and the open reading frame of beta-amyrin synthase was cloned by RT-PCR strategy with the template of total RNA extracted from roots of G. uralensis.</p><p><b>RESULT</b>The GubAS (GenBank Accession number FJ627179) was 2 289 bp in length encoding one pelypeptide of 762 amino acid. Deduced amino acid sequence had 99%, 92%, 90%, 90% and 89% homology to the amino acid sequence of G. glabra, Lotus japonicus, Pisum sativum, Medicago truncatula, Glycine max, respectively.</p><p><b>CONCLUSION</b>The open reading frame of bAS from G. uralensis is cloned and reported for the first time. The conclusion will provide a foundation for exploring the mechanism of triterpenes biosynthesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Plant Proteins
/
Plants
/
Molecular Sequence Data
/
Open Reading Frames
/
Classification
/
Cloning, Molecular
/
Intramolecular Transferases
/
Glycyrrhiza uralensis
/
Genetics
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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