Optimization of pre-coated multi-probe fluorescence in situ hybridization for cytogenetic detection of acute leukemia / 南方医科大学学报
Journal of Southern Medical University
; (12): 1457-1460, 2012.
Article
in Zh
| WPRIM
| ID: wpr-315442
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To optimize pre-coated multiple-probe fluorescence in situ hybridization (FISH) to improve its efficiency in cytogenetic diagnosis of acute leukemia.</p><p><b>METHODS</b>The original multiple-probe FISH techniques were optimized by adjusting the cell density and adding a process of protease digestion. Cytogenetic anomalies were detected in 141 patients with acute lymphocytic leukemia (ALL) or acute myeloid leukemia/ myelodysplastic syndromes (AML/MDS) using the modified technique, and 35 of the patients were also examined using the original technique. The successful detection rate and positive site detection rate were compared between the modified and original techniques.</p><p><b>RESULTS</b>Modification of the pre-coated multiple-probe FISH technique resulted in an significant increase of the successful detection rate (from 85.3% to 100%) and the positive site detection rate (from 5.1% to 8.6%) in ALL patients; in AML/MDS patients, the successful detection rate was significantly improved from 67.4% to 99.8% and the positive site detection rate from 3.5% to 6.0% (P<0.01).</p><p><b>CONCLUSION</b>The modified pre-coated multiple-probe FISH technique can significantly increase the diagnostic efficiency of cytogenetic abnormalities in leukemic patients.</p>
Full text:
1
Index:
WPRIM
Main subject:
Myelodysplastic Syndromes
/
Leukemia, Myeloid, Acute
/
Chromosome Aberrations
/
In Situ Hybridization, Fluorescence
/
Diagnosis
/
Genetics
/
Karyotyping
/
Methods
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article