Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1250-1254, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-315490
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1).</p><p><b>METHODS</b>Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR.</p><p><b>RESULTS</b>The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects.</p><p><b>CONCLUSION</b>Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Protein Kinase C
/
Thrombin
/
Cell Line
/
Cells, Cultured
/
Chemokine CCL2
/
Cell Biology
/
Fibroblasts
/
Indoles
/
Lung
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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