Effect of silicosis alveolar macrophages on expression of c-myc in human embryo lung fibroblast in vitro / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 468-471, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-315664
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of silicosis alveolar macrophages (AM) restimulated by SiO(2) on expression of c-myc oncogene in human embryo lung fibroblasts.</p><p><b>METHODS</b>The bronchoalveolar lavage of silicosis patients was collected. AMs were divided into 2 groups (1) SiO(2) AMs were stimulated with SiO(2) (30 microg/ml) for 1, 2, 6, 12, 24 and 36 h; (2) control treated for the same time without SiO(2). Fibroblasts were cultured with different AMs supernatants for 2 h or 7 h respectively. The expression of c-myc mRNA was determined by RT-PCR and protein by Western Blot.</p><p><b>RESULTS</b>There was no c-myc expression when fibroblasts were static. The supernatants in the S6 group stimulated expression of c-myc mRNA and protein, with the peak expression at 2 h and 7 h respectively. In the control group, AMs supernatants cultured in different time stimulated expression of c-myc mRNA and protein with the most evident expression at 12 h. The ratios were 0.749 +/- 0.088 and 0.759 +/- 0.101 respectively. Compared with control in the same period, c-myc mRNA and protein expression were significantly stronger treated with the supernatants in which AMs were stimulated for 1 h, 2 h and 6 h by SiO(2) (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>AMs stimulated with SiO(2) has the ability to induce c-myc oncogene expression in human embryo lung fibroblasts.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Silicosis
/
RNA, Messenger
/
Cells, Cultured
/
Proto-Oncogene Proteins c-myc
/
Macrophages, Alveolar
/
Silicon Dioxide
/
Toxicity
/
Fibroblasts
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2008
Type:
Article
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