Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecipitation / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To detect and verify activator protein-1 (AP-1) binding to p53 gene promoter region in A549 cell lines in vitro.</p><p><b>METHODS</b>AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation (ChIP) assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment.</p><p><b>RESULTS</b>The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody.</p><p><b>CONCLUSION</b>AP-1 binds to p53 gene promoter region in A549 cells, and regulates its expression.</p>