Effects of gossypol acetic acid on the proliferation and methylation level of the human MutL homologue 1 gene in human tongue carcinoma cell line Tca8113 / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 91-95, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-315867
ABSTRACT
<p><b>OBJECTIVE</b>This paper aims to study the effects of gossypol acetic acid (GAA) on proliferation and methylation level of human MutL homologue 1 (hMLH1) gene in human tongue cancer cell line Tca8113.</p><p><b>METHODS</b>The MTT assay was used to determine the effects of the acid on the proliferation inhibition in Tca8113 cells treated with different GAA concentrations. Nested methylation-specific polymerase chain reaction (nMSP) was used to detect the change in the methylation level of hMLH1 after 48 and 72 h with 30 and 15 micro mol L(-1) GAA treatment.</p><p><b>RESULTS</b>MTT assay results showed the growth and proliferation inhibition of Tca8113 cells in the experimental GAA group after 24 h to 72 h of GAA treatment. The nMSP results indicated that the average optical density of hMLH1 in the Tca8113 cells significantly changed after the GAA treatment (30 micro mol L(-1) GAA for 48 h and 15 micro mol L(-1) for 72 h) (P<0.05) compared with that of the control group.</p><p><b>CONCLUSION</b>GAA does not only inhibit Tca8113 proliferation but also has a demethylation effect on the hMLH1 gene. These phenomena may be part of an underlying tumor-suppression mechanism of GAA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gossypol
/
Tongue Neoplasms
/
Cell Line
/
Cell Line, Tumor
/
Cell Proliferation
/
Methylation
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2014
Type:
Article
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