Effect of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension culture / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1580-1583, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-316003
ABSTRACT
<p><b>OBJECTIVE</b>To illustrate the effects of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures.</p><p><b>METHOD</b>High performance liquid chromatography and plant tissue culture were applied.</p><p><b>RESULT AND CONCLUSION</b>Cell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration. Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isoflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Polystyrenes
/
Polyvinyls
/
Sucrose
/
Cells, Cultured
/
Charcoal
/
Culture Media
/
Pueraria
/
Cell Biology
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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