Copy Number Alterations of BCAS1 in Squamous Cell Carcinomas
Korean Journal of Pathology
;
: 271-275, 2011.
Article
in English
| WPRIM
| ID: wpr-31609
ABSTRACT
BACKGROUND:
Breast carcinoma amplified sequence 1 (BCAS1), located in 20q13, is amplified and overexpressed in breast cancers. Even though BCAS1 is expected to be an oncogene candidate, its contribution to tumorigenesis and copy number status in other malignancies is not reported. To elucidate the role of BCAS1 in squamous cell carcinomas, we investigated the copy number status and expression level of BCAS1 in several squamous cell carcinoma cell lines, normal keratinocytes and primary tumors.METHODS:
We quantitated BCAS1 gene by real-time polymerase chain reaction (PCR). Expression level of BCAS1 was measured by real-time reverse transcription-PCR and immunoblot.RESULTS:
Seven (88%) of 8 squamous cell carcinoma cell lines showed copy number gain of BCAS1 with various degrees. BCAS1 gene in primary tumors (73%) also showed copy number gain. However, expression level did not show a linear correlation with copy number changes.CONCLUSIONS:
We identified copy number gain of BCAS1 in squamous cell carcinomas. Due to lack of linear correlation between copy numbers of BCAS1 and its expression level, we could not confirm that the overexpression of BCAS1 is a common finding in squamous cell carcinoma cell lines. However, this study shows that the copy number gain of BCAS1 is a common finding in squamous cell carcinomas.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Breast
/
Carcinoma, Squamous Cell
/
Gene Expression
/
Keratinocytes
/
Cell Line
/
Cell Transformation, Neoplastic
/
Gene Dosage
/
Coat Protein Complex I
/
DNA Copy Number Variations
Type of study:
Prognostic study
Language:
English
Journal:
Korean Journal of Pathology
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS