Relationship between the expression of human leukocyte antigen-b in peripheral blood leukocytes and the differentiation and metastasis of gastric carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 49-52, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-316247
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between the expression of host human leukocyte antigen-B mRNA (HLA-B mRNA) and HLA-B antigen in peripheral blood leukocytes (PBLs) and the differentiation and metastasis of gastric carcinoma (GC).</p><p><b>METHODS</b>To design and screen specific primers of HLA-B gene independently, detect the expression of HLA-B mRNA from 30 GC patients by reverse transcription-PCR and compare with the HLA-B antigen expression measured by flow cytometry.</p><p><b>RESULTS</b>The expression rate of PBL HLA-B mRNA from GC patients (23. 3% ) was very significantly lower than that of normals (87. 5% ) (P <0. 01) , especially concerning the poorly differentiated GC patients with lymph node metastasis (16. 0% ). Measured by flow cytometry, the expression percentage of HLA-B antigen of well-differentiated GC patients without lymph node metastasis was 88. 2% , an obviously decreasing tendency was showed in comparison with that in the normal group (98. 8% ) , although the difference was not significant (P = 0. 056) , and the expression percentage in poorly differentiated GC patients with lymph node metastasis(73. 3% )was declined significantly (P <0. 05).</p><p><b>CONCLUSION</b>The expression of PBL HLA-B mRNA and HLA-B antigen in GC patients is decreased or lost, and correlated with differentiation and metastasis of the cancer. The expression of PBL HLA-B mRNA may more directly reflect its relationship with the tumor differentiation and metastasis than that of HLA-B antigen.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Stomach Neoplasms
/
RNA, Messenger
/
HLA-B Antigens
/
Gene Expression Regulation, Neoplastic
/
Cell Differentiation
/
Reverse Transcriptase Polymerase Chain Reaction
/
Allergy and Immunology
/
Flow Cytometry
/
Genetics
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article
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