Cytogenetic and interphase FISH studies in the diagnosis of malignant lymphomas / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 45-48, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-316248
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the role of cytogenetic study and interphase FISH analysis in differential diagnosis of patients with clinical and/or cytological diagnosis as lymphoma or "suspicious for lymphoma".</p><p><b>METHODS</b>Routine histology, immunohistochemistry, cytogenetics and interphase FISH studies were used to assess 223 cases with superficial lymph nodes of not less than 1. 5 cm in diameter. The probe used in the interphase FISH assays is the Vysis' LSI IGH Dual Color, Break Apart Rearrangement Probe.</p><p><b>RESULTS</b>Based on these studies, forty-four patients were diagnosed as Hodgkin's lymphomas ( HL) , 162 as Non-Hodgkin's lymphomas ( NHL) , 11 with benign diseases and 4 as other malignancies, while the remaining 2 cases were discarded due to tissue necrosis. Using interphase FISH, abnormalities of immunoglobulin heavy chain gene (IGH) were detected in 6/44 (13.6%) and 83/162 (51.2%) in the HIL and NHL cases, respectively, while none was observed in 11 cases with a benign disease (P <0. 001). Combining cytogenetics and FISH studies, the detection rates for HL and NHL cases then increased to 15.9% and 77. 8%, respectively, otherwise, 3 of whom could not have made definite diagnosis.</p><p><b>CONCLUSION</b>Interphase FISH assay is a rapid and sensitive tool for detecting IGH abnormalities. Both cytogenetics and interphase FISH analyses may play a significant role in diagnosis of lymphomas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sarcoidosis
/
Lymphoma, Non-Hodgkin
/
Hodgkin Disease
/
Immunohistochemistry
/
Chromosome Aberrations
/
In Situ Hybridization, Fluorescence
/
Pseudolymphoma
/
Proto-Oncogene Proteins c-bcl-2
/
Cytogenetic Analysis
/
Diagnosis
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article
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