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Adenovirus-delivered tissue inhibitor of metalloproteinases-3 transfection increases the sensitivity of cervical cancer cells to cisplatin / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 25-29, 2007.
Article in Chinese | WPRIM | ID: wpr-316252
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of adenovirus-delivered tissue inhibitor of metalloproteinases-3 ( Ad-TIMP-3) on sensitivity of cervical cancer cells to cisplatin and evaluate the potential application of this combined scheme in cervical cancer treatment.</p><p><b>METHODS</b>Cells of cervical cancer CaSKi cell line were infected with Ad-TIMP-3 in vitro. Apoptotic effect, cell cycle changes and p53 protein expression were detected. After combined treatment of those cells with cisplatin, colony formation test was performed and cytotoxicity was detected by MTT. The growth curve and tumor growth inhibition in vivo were evaluated.</p><p><b>RESULTS</b>The expressions of TIMP-3 mRNA and protein were significantly upregulated after transfection. As a result, massive apoptosis was induced and the cells were arrested at G2/M phase. Exogenous overexpression of TIMP-3 increased p53 protein level markedly in spite of the backgrounds of p53 gene in cells. Combined with cisplatin treatment, the cloning efficiency was decreased. A synergism was observed by isobolic method ( D < 1 ) in vitro and tumor growth was significantly inhibited in vivo.</p><p><b>CONCLUSION</b>Ad-TIMP-3 is a powerful proapoptotic agent. It increases sensitivity of the cells to cisplatin and the Ad-TIMP-3 gene therapy in combination with cisplatin could be a promising alternative in cervical cancer treatment.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Therapeutics / RNA, Messenger / HeLa Cells / Carcinoma, Squamous Cell / Transfection / Genetic Therapy / Random Allocation / Cell Cycle Type of study: Controlled clinical trial Limits: Animals / Female / Humans Language: Chinese Journal: Chinese Journal of Oncology Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Therapeutics / RNA, Messenger / HeLa Cells / Carcinoma, Squamous Cell / Transfection / Genetic Therapy / Random Allocation / Cell Cycle Type of study: Controlled clinical trial Limits: Animals / Female / Humans Language: Chinese Journal: Chinese Journal of Oncology Year: 2007 Type: Article