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Representative appressorium stage cDNA library of Magnaporthe grisea / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B ; (12): 132-136, 2005.
Article in English | WPRIM | ID: wpr-316363
ABSTRACT
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA, Fungal / Gene Expression Regulation, Fungal / Gene Library / Cloning, Molecular / Sequence Analysis, DNA / Magnaporthe / Gene Expression Profiling / Genetics / Methods Language: English Journal: Journal of Zhejiang University. Science. B Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA, Fungal / Gene Expression Regulation, Fungal / Gene Library / Cloning, Molecular / Sequence Analysis, DNA / Magnaporthe / Gene Expression Profiling / Genetics / Methods Language: English Journal: Journal of Zhejiang University. Science. B Year: 2005 Type: Article