Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 74-78, 2005.
Article
in English
| WPRIM
| ID: wpr-316373
ABSTRACT
The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns (S,S)-Whelk-O 1 and ChiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on ChiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S,S)-Whelk-O 1 column with hexane-ethanol (973, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on ChiraSpher column with hexane-ethanol-THF-diethylamine (92350.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Piperidines
/
Pyridines
/
Mass Spectrometry
/
Butylene Glycols
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Chemical Fractionation
/
Isomerism
/
Acetamides
/
Methods
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2005
Type:
Article
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