Neuroprotective Effects of Intraischemic(1 hour) Moderate Hypothermia in Gerbil Brain Global Ischemic Model
Journal of the Korean Society of Emergency Medicine
;
: 350-362, 1999.
Article
in Korean
| WPRIM
| ID: wpr-31652
ABSTRACT
BACKGROUND:
In animal models of cerebral ischemic-reperfusion has been shown to have a beneficial effect. The object of this study is to compare the effect of pathologic findings between normotheimic and moderate hypothermic group.METHODS:
We investigated the effect of moderate hypothermia induced 1 hour after transient(10 min) both carotid artery occlusion on the extent of ischemic-reperfusion cell damage in Mongolian Gerbil model. The terminal deoxyribonucleotidyl transferase (TdT) -mediated biotin-16-dUTP nick-end labelling(TUNEL staning) are used to detect apoptosis.RESULTS:
1. We suggest that Core body temperature is down to moderate hypothermia(30-32degrees C) beyond 10 minite by selective bain cooling method in Mongolian Gerbil model. 2. By light microscopy, ischemic-reperfusion damage were detected in the hippocampal CA1 pyramidal layer on the 3 day after transient ischemic insult, which showed chrosomal condensation and cytoplasmic eosinophilia. Ischemic-reperfusion cells were increased in the CA1 region on the 5 day. Apoptotic cells of the CA1 neurons seen by TUNEL staining than ischemic neurons seen by Hematoxylin-eosin staining were investigate 3 and 5 days after ischemic-reperfusion insult.CONCLUSION:
We suggest that is not neuroprotective effects of Intraischemic(1 hour) moderate hypothermia in Gerbil brain global ischemic-reperfusion model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Body Temperature
/
Brain
/
Carotid Arteries
/
Gerbillinae
/
Apoptosis
/
Neuroprotective Agents
/
In Situ Nick-End Labeling
/
Models, Animal
/
Cytoplasm
/
DNA Nucleotidylexotransferase
Type of study:
Prognostic study
Language:
Korean
Journal:
Journal of the Korean Society of Emergency Medicine
Year:
1999
Type:
Article
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