Effects of GDP on the activity and expression of mitochondrial uncoupling proteins in rat brain in vitro / 生理学报
Acta Physiologica Sinica
;
(6): 492-496, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-316700
ABSTRACT
To investigate the regulatory role of purine nucleotide on uncoupling proteins (UCPs), the activity of UCPs and the expressions of UCP4 and UCP5 in mitochondria of rat brain tissues incubated with GDP were observed in vitro. The cerebral hemispheres of adult male Sprague-Dawley rats were removed and clipped into 8-10 mm3 tissue mass which incubated with 1 mmol/L GDP (GDP group), or only incubation medium (control group), for 30 min in vitro. The mitochondria from incubated tissue mass of rat brain were isolated by centrifugation. The activity of UCPs was detected by the method of [3H]-GTP binding with UCPs specifically. The maximal binding content (Bmax) and the dissociation constant (Kd) were determined from Scatchard plot. The mRNA and protein expressions of UCP4 and UCP5 were measured by RT-PCR and Western blot, respectively. The results showed that Bmax was increased and Kd was decreased in rat brain mitochondria in GDP group compared with that in control group. But the mRNA and protein expressions of UCP4 and UCP5 exhibited no statistically significant changes. It is thus suggested that GDP inhibits the activity of mitochondrial UCPs in rat brain in vitro, but exhibits no effect on the expressions of UCP4 and UCP5.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
In Vitro Techniques
/
Brain
/
RNA, Messenger
/
Rats, Sprague-Dawley
/
Reverse Transcriptase Polymerase Chain Reaction
/
Mitochondrial Proteins
/
Mitochondrial Membrane Transport Proteins
/
Mitochondrial Uncoupling Proteins
/
Guanosine Diphosphate
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2008
Type:
Article
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