Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3 / 生理学报
Acta Physiologica Sinica
;
(6): 391-396, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-316714
ABSTRACT
The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells from 9 day-old rat in vitro. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense c-src oligodeoxynucleotides (ODNs) in vitro; RT-PCR was utilized to observe the expression of c-src mRNA and Western blot was used to observe the protein expressions of pp60c-src and phosphorylated signal transducer and activator of transcription-3 (p-STAT3). Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.1% (P<0.05) and the expression of c-src mRNA decreased significantly after treatment with 10 μmol/L antisense c-src ODNs for 12 h. Compared with that in the control group, the protein expressions of pp60c-src and p-STAT3 decreased by 33.8% and 45.3% (both P<0.01), respectively, in the spermatogonial stem cells after being transfected with antisense c-src ODNs. The results suggest that proto-oncogene c-src regulates the viability of rat spermatogonial stem cells through p-STAT3.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Spermatogonia
/
Stem Cells
/
RNA, Messenger
/
Transfection
/
Cells, Cultured
/
Genes, src
/
Proto-Oncogene Proteins pp60(c-src)
/
Cell Biology
/
STAT3 Transcription Factor
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2008
Type:
Article
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